Utility of Serum miR-125b as a Diagnostic and Prognostic Indicator and Its Alliance with a Panel of Tumor Suppressor Genes in Epithelial Ovarian Cancer.

摘要: MicroRNAs (miRNAs) have been found to be dysregulated in epithelial ovarian cancer (EOC) and may function as either tumor suppressor genes (TSGs) or oncogenes. Hypermethylation of miRNA silences the tumour suppressive a hypermethylation TSG regulating that (or vice versa) leads its loss function. The present study aims evaluate impact aberrant microRNA-125b (miR-125b) expression on various clinicopathological features association with anomalous methylation several TSGs. We enrolled 70 newly diagnosed cases cancer, recorded their clinical history healthy female volunteers. Serum miR-125b levels were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) status TSGs was investigated specific PCR. ROC curves constructed estimate diagnostic prognostic usefulness miR-125b. Kaplan-Meier method applied compare survival curves. Expression significantly upregulated (p<0.0001) comparison controls. level associated FIGO stage, lymph node distant metastasis. curve for potential yielded significant AUC an equitable sensitivity specificity. prognosis AUCs histological grade, distal metastasis, survival. also correlated Our results indicate DNA involved inactivation independent biomarker outcome EOC.