Gold nanoparticle-based detection of genomic DNA targets on microarrays using a novel optical detection system
作者： James J Storhoff , Sudhakar S Marla , Paul Bao , Susan Hagenow , Hitesh Mehta
DOI: 10.1016/J.BIOS.2003.08.014
关键词: Nanotechnology 、 Complementary DNA 、 genomic DNA 、 Biology 、 Nanoparticle 、 Colloidal gold 、 Biosensor 、 Oligonucleotide 、 DNA microarray 、 DNA
摘要: Abstract The development of a nanoparticle-based detection methodology for sensitive and specific DNA-based diagnostic applications is described. technology utilizes gold nanoparticles derivatized with thiol modified oligonucleotides that are designed to bind complementary DNA targets. A glass surface arrays immobilized oligonucleotide capture sequences used targets, which then detected via hybridization the nanoparticle probes. Amplification silver allows quantitation by measuring evanescent wave induced light scatter low-cost optical systems. Compared Cy3-based fluorescence, amplified probes provide ∼1000-fold increase in sensitivity. Furthermore, direct non-amplified genomic from infectious agents afforded through increased specificity even identification single nucleotide polymorphisms (SNP) human appears feasible.
elsevier.com
sci-hub.se 参考文章(28)
David A Lane, Pier M Mannucci, Kenneth A Bauer, Rogier M Bertina, Nikolay P Bochkov, Victor Boulyjnkov, Mammen Chandy, Bjorn Dahlback, E K Ginter, Joseph P Miletich, Frits R Rosendaal, Uri Seligsohn, Inherited Thrombophilia: Part 1 Thrombosis and Haemostasis. ,vol. 76, pp. 651- 662 ,(1996) , 10.1055/S-0038-1650638
Rogier M. Bertina, Bobby P. C. Koeleman, Ted Koster, Frits R. Rosendaal, Richard J. Dirven, Hans de Ronde, Pieter A. van der Velden, Pieter H. Reitsma, Mutation in blood coagulation factor V associated with resistance to activated protein C Nature. ,vol. 369, pp. 64- 67 ,(1994) , 10.1038/369064A0
YunWei Charles Cao, Rongchao Jin, Chad A Mirkin, Nanoparticles with Raman Spectroscopic Fingerprints for DNA and RNA Detection Science. ,vol. 297, pp. 1536- 1540 ,(2002) , 10.1126/SCIENCE.297.5586.1536
James J. Storhoff, Robert Elghanian, Robert C. Mucic, Chad A. Mirkin, Robert L. Letsinger, One-Pot Colorimetric Differentiation of Polynucleotides with Single Base Imperfections Using Gold Nanoparticle Probes Journal of the American Chemical Society. ,vol. 120, pp. 1959- 1964 ,(1998) , 10.1021/JA972332I
Robert Elghanian, James J. Storhoff, Robert C. Mucic, Robert L. Letsinger, Chad A. Mirkin, Selective Colorimetric Detection of Polynucleotides Based on the Distance-Dependent Optical Properties of Gold Nanoparticles Science. ,vol. 277, pp. 1078- 1081 ,(1997) , 10.1126/SCIENCE.277.5329.1078
Ian D. Walton, Scott M. Norton, Arjuna Balasingham, Lin He, Dominador F. Oviso, Dimpy Gupta, Paul A. Raju, Michael J. Natan, R. Griffith Freeman, Particles for multiplexed analysis in solution: detection and identification of striped metallic particles using optical microscopy. Analytical Chemistry. ,vol. 74, pp. 2240- 2247 ,(2002) , 10.1021/AC020073W
Robert J. Lipshutz, Stephen P.A. Fodor, Thomas R. Gingeras, David J. Lockhart, High density synthetic oligonucleotide arrays Nature Genetics. ,vol. 21, pp. 20- 24 ,(1999) , 10.1038/4447
C. S. Brown, P. C. Goodwin, P. K. Sorger, Image metrics in the statistical analysis of dna microarray data Proceedings of the National Academy of Sciences of the United States of America. ,vol. 98, pp. 8944- 8949 ,(2001) , 10.1073/PNAS.161242998
Angela Relógio, Christian Schwager, Alexandra Richter, Wilhelm Ansorge, Juan Valcárcel, Optimization of oligonucleotide-based DNA microarrays. Nucleic Acids Research. ,vol. 30, ,(2002) , 10.1093/NAR/30.11.E51
T. Andrew Taton, Gang Lu, Chad A. Mirkin, Two-color labeling of oligonucleotide arrays via size-selective scattering of nanoparticle probes. Journal of the American Chemical Society. ,vol. 123, pp. 5164- 5165 ,(2001) , 10.1021/JA0102639