Glucansucrases: three-dimensional structures, reactions, mechanism, α-glucan analysis and their implications in biotechnology and food applications.

作者: Hans Leemhuis , Tjaard Pijning , Justyna M. Dobruchowska , Sander S. van Leeuwen , Slavko Kralj

DOI: 10.1016/J.JBIOTEC.2012.06.037

关键词: Lactic acidGlucansucraseLactobacillalesBiotechnologyProtein engineeringBiologyBiochemistryAlternansucrasePrebioticFood technologyGlucan

摘要: Glucansucrases are extracellular enzymes that synthesize a wide variety of α-glucan polymers and oligosaccharides, such as dextran. These carbohydrates have found numerous applications in food health industries, can be used pure compounds or even produced situ by generally regarded safe (GRAS) lactic acid bacteria applications. Research the recent years has resulted big steps forward understanding exploitation biocatalytic potential glucansucrases. This paper provides an overview glucansucrase enzymes, their recently elucidated crystal structures, reaction product specificity, structural analysis polymers. Furthermore, we discuss key developments polymer formation based on three-dimensional structures proteins. Finally (potential) α-glucans related industries.

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