All-printed semiquantitative paper-based analytical devices relying on QR code array readout

摘要: The wide spread of smartphones and QR codes for various end-user applications has had an impact beyond traditional fields use, recently also reaching point-of-care testing (POCT). This work presents the integration code recognition into paper-based analytical devices (PADs) with “distance-based” colorimetric signalling, resulting in semiquantitative readout fully relying on straightforward barcode reader solutions. PADs consist array arranged series inside a paperfluidic channel. A mask dye concept been developed, which enables utilisation colour changing indicators by initially hiding codes. change indicator induced presence analyte interest results gradual unmasking codes, become recognisable smartphone app. To reproducibly fabricate devices, all fabrication steps were performed commercial desktop solid ink inkjet printing. masking function was optimised controlling amount printed through adjusting opacity printing patterns during deposition process. For proof-of-concept, model assay form copper ion (Cu2+) detection concentration range 0.4 mM to 3.2 evaluated. Consistent independent environmental light condition achieved free best our knowledge, this is first demonstration approach without digital analysis, customised app or hardware modification.