Clinicopathological significance of the CRTC3-MAML2 fusion transcript in mucoepidermoid carcinoma.

摘要: Mucoepidermoid carcinoma is the most common primary malignancy of salivary gland. We and others showed that CRTC1-MAML2 gene fusion was associated with favorable clinicopathological tumor features. Recently, a novel fusion, CRTC3-MAML2, reported as rare alteration in case mucoepidermoid carcinoma. However, its frequency significance remains unclear. In all, 101 cases 89 non-mucoepidermoid gland were analyzed, RNA extracted from formalin-fixed, paraffin-embedded specimens. CRTC family, there have been three genes, CRTC1, CRTC2, CRTC3. developed reverse transcription-polymerase chain reaction (RT-PCR) assays for CRTC1-MAML2, CRTC2-MAML2, CRTC3-MAML2 fusions. Clinicopathological data patients obtained their clinical records. Of carcinoma, 34 (34%) 6 (6%) positive transcripts. neither transcript noted. former cases, fusions mutually exclusive. The other not detected. confirmed features CRTC1-MAML2-positive carcinomas indicated an indolent course. CRTC3-MAML2-positive also had clinicopathologically features; all less advanced stage, negative nodal metastasis, no high-grade histology, recurrence or tumor-related death after surgical resection tumor. It interesting to note tumors (mean 36 years age) significantly younger those (55 years) fusion-negative (58 years). conclusion, which exclusive specific may be detected more frequently than previously expected. possessing detectable fusion.