Simultaneous quantification of catecholamines in rat brain by high-performance liquid chromatography with on-line gold nanoparticle-catalyzed luminol chemiluminescence detection.

摘要: A new method based on high-performance liquid chromatography (HPLC) coupled with on-line gold nanoparticle-catalyzed luminol chemiluminescence (CL) detection was developed for the simultaneous quantitation of catecholamines in rat brain. In present CL system, nanoparticles were produced by reaction H2O2, NaHCO3−Na2CO3 (buffer solution luminol) and HAuCl4. Norepinephrine (NE), epinephrine (EP) dopamine (DA) could strongly enhance signal system. The UV−visible absorption spectra transmission electron microscopy studies carried out, enhancement mechanism proposed. Catecholamines promoted formation more nanoparticles, which better catalyzed luminol–H2O2 reaction. good separation NE, EP DA achieved isocratic elution using a mixture methanol 0.2% aqueous phosphoric acid (5:95, v/v) within 8.5 min. Under optimized conditions, limits, defined as signal-to-noise ratio 3, range 1.32–1.90 ng/mL, corresponding to 26.4−38.0 pg 20 μL sample injection. recoveries added brain >94.6%, precisions <5.5%. validated HPLC−CL successfully applied determine NE without prior purification. Copyright © 2014 John Wiley & Sons, Ltd.