Analysis of catecholamines and their metabolites in adrenal gland by liquid chromatography tandem mass spectrometry

摘要: Abstract Two simple, rapid and specific analytical methods for 13 catecholamines their metabolites have been developed based on liquid chromatography tandem mass spectrometry in a multiple reaction monitoring mode. Tyrosine, dopamine, dihydroxyphenylalanine, epinephrine, norepinephrine, 3-methoxytyramine, normetanephrine, metanephrine isoproterenol (internal standard) were separated Kromasil™ Cyano column by mobile phase consisting of 60% (v/v) acetonitrile 40% water adjusted with formic acid to pH 3.0, detected positive ionization electrospray spectrometry. While vanillymandelic acid, 3,4-dihydroxymandelic homovanillic 3,4-dihydroxyphenylacetic 4-hydroxy-3-methoxyphenylglycol 5-hydroxy-2-indolecarboxylic reversed-phase Shim-Pak VP-ODS the acetonitrile, 4.5 negative The influence various parameters such as type composition separation sensitivity investigated. limits detection range 0.5–20 ng mL−1. mean recoveries determined from three different concentrations each analyte above 85.4%. precision method calculated relative standard deviation was lower than 5.3%. Deduced results real sample analysis, adrenal gland synthesizes stores catecholamine hormones norepinephrine epinephrine.