Embryo multinucleation at the two-cell stage is an independent predictor of intracytoplasmic sperm injection outcomes

摘要: Objective To determine the prognostic impact of nuclear status at two-cell stage on intracytoplasmic sperm injection (ICSI) outcomes. Design Retrospective study. Setting Hospital. Patient(s) Only ICSI cycles with time-lapse monitoring transferred embryos known implantation/delivery data from November 2012 to December 2014 were included. A total 2,449 assessed for multinucleation rates two- and four-cell stage, 608 studied Intervention(s) None. Main Outcome Measure(s) Implantation rate (IR) live birth (LBR) according number multinucleated blastomeres stage: none (Without-MNB 2cell ), one (MNB 1/2cell two 2/2cell ); morphokinetics MNB embryos. Result(s) Embryos led lower IR (27.7%) LBR (22.7%) than Without-MNB (33.4% 29.8%, respectively). The significantly (18.3%) (13.4%) . This difference remained significant in multivariate analysis implantation (odds ratio 0.57; 95% confidence interval 0.34–0.94) 0.46; 0.26–0.80), independently other parameters (women's age, time formation, stage). Among implanted , if cleavage into four cells occurred later 37 hours after insemination, more likely lead birth. Conclusion(s) presence specifically both had a negative potential. Thus, embryo should be used as an additional noninvasive criterion selection.