Ligand-guided-selection method for screening antigen-specific ligands
作者： Prabodhika Mallikaratchy
DOI:
关键词: Systematic evolution of ligands by exponential enrichment 、 Aptamer 、 Biology 、 Antibody 、 Selection method 、 Ligand 、 Antigen 、 Antigen specific 、 Combinatorial chemistry
摘要: A Ligand-guided-Selection (LIGS) method for identifying highly specific aptamers against a predetermined antigen of target is provided. LIGS uses stronger and bivalent binder (e.g. an antibody) interacting with its cognate to displace from partially enriched SELEX pool. Elution the displaced provides that are antigen.
lens.org 参考文章(16)
David Spetzler, Tassilo Hornung, Valeriy Domenyuk, Frank Schafer, David Halbert, Nianqing Xiao, Aptamers and uses thereof ,(2013)
Larry Gold, Rebecca Vanderslice, Daniel J. Schneider, Flow cell selex ,(1997)
Matthew Wecker, Kirk Jensen, Bruce Eaton, Larry Gold, Drew Smith, Systematic evolution of ligands by exponential enrichment: tissue SELEX ,(1996)
Jonathan Drew Smith, Dominic A. Zichi, Larry Gold, Modified SELEX processes without purified protein ,(2002)
Donald H Burke, Larry Gold, RNA aptamers to the adenosine moiety of S-adenosyl methionine: structural inferences from variations on a theme and the reproducibility of SELEX Nucleic Acids Research. ,vol. 25, pp. 2020- 2024 ,(1997) , 10.1093/NAR/25.10.2020
Kwame Sefah, Dihua Shangguan, Xiangling Xiong, Meghan B O'Donoghue, Weihong Tan, Development of DNA aptamers using Cell-SELEX Nature Protocols. ,vol. 5, pp. 1169- 1185 ,(2010) , 10.1038/NPROT.2010.66
Mao Ye, Jun Hu, Minyuan Peng, Jing Liu, Jun Liu, Huixia Liu, Xielan Zhao, Weihong Tan, Generating Aptamers by Cell-SELEX for Applications in Molecular Medicine International Journal of Molecular Sciences. ,vol. 13, pp. 3341- 3353 ,(2012) , 10.3390/IJMS13033341
Günter Mayer, Marie-Sophie L Ahmed, Andreas Dolf, Elmar Endl, Percy A Knolle, Michael Famulok, Fluorescence-activated cell sorting for aptamer SELEX with cell mixtures Nature Protocols. ,vol. 5, pp. 1993- 2004 ,(2010) , 10.1038/NPROT.2010.163
D. Shangguan, Y. Li, Z. Tang, Z. C. Cao, H. W. Chen, P. Mallikaratchy, K. Sefah, C. J. Yang, W. Tan, Aptamers evolved from live cells as effective molecular probes for cancer study Proceedings of the National Academy of Sciences of the United States of America. ,vol. 103, pp. 11838- 11843 ,(2006) , 10.1073/PNAS.0602615103
Samantha E Wilner, Brian Wengerter, Keith Maier, Maria de Lourdes Borba Magalhães, David Soriano Del Amo, Supriya Pai, Felipe Opazo, Silvio O Rizzoli, Amy Yan, Matthew Levy, An RNA Alternative to Human Transferrin: A New Tool for Targeting Human Cells Molecular therapy. Nucleic acids. ,vol. 1, ,(2012) , 10.1038/MTNA.2012.14