Comparative proteomic analysis of a potentially probiotic Lactobacillus pentosus MP-10 for the identification of key proteins involved in antibiotic resistance and biocide tolerance

摘要: Probiotic bacterial cultures require resistance mechanisms to avoid stress-related responses under challenging environmental conditions; however, understanding these traits is required discern their utility in fermentative food preparations, versus clinical and agricultural risk. Here, we compared the proteomic of Lactobacillus pentosus MP-10, a potentially probiotic lactic acid bacteria isolated from brines naturally fermented Alorena green table olives, exposed sub-lethal concentrations antibiotics (amoxicillin, chloramphenicol tetracycline) biocides (benzalkonium chloride triclosan). Several genes became differentially expressed depending on antimicrobial exposure, such as up-regulation protein synthesis, down-regulation carbohydrate metabolism energy production. The antimicrobials appeared have altered Lb. MP-10 physiology achieve gain cellular for survival. For example, biocide-adapted exhibited down-regulated phosphocarrier HPr an unexpressed oxidoreductase. However, synthesis was over-expressed antibiotic- cells (ribosomal proteins glutamyl-tRNA synthetase), possibly compensate damaged targeted by antimicrobials. Furthermore, stress proteins, NADH peroxidase (Npx) small heat shock protein, were only antibiotic-adapted MP-10. Results showed that adaptation could be good way desirable robustness various gastrointestinal conditions (e.g., bile stresses).