Proteomic analysis of Lactobacillus pentosus for the identification of potential markers involved in acid resistance and their influence on other probiotic features

摘要: Acidity often prevents the undesirable microbial colonization both in fermented foods and under gastric conditions. Thus, acid resistance of Lactobacillus pentosus strains used as starter cultures and/or probiotics requires further understanding. This was investigated by means comparative proteomic approach using three representing phenotypes: resistant (AP2-15), intermediate (AP2-18) sensitive (LP-1) to acidic Proteomic analysis constitutive phenotypes revealed that intrinsic L. is associated with over-production principal proteins: 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase 2 (PGAM-d), elongation factor G 50S ribosomal protein L10, additionally on ATP synthase subunit beta chaperone DnaK; they are metabolic pathways proteins carbohydrates, energy production stress responses. Suggested biomarkers for include PGAM-d, being abundantly found proteome phenotype standard Furthermore, pre-exposed acids displayed enhanced probiotic function such auto-aggregation ability via surface proteins. We conclude pre-exposure may strategically enhance their performance probiotics.