miR-423-5p inhibits the proliferation and metastasis of glioblastoma cells by targeting phospholipase C beta 1.

摘要: Glioma is a common brain tumor which highly invasive, responds poorly to therapy, and has poor prognosis. There growing evidence that an abnormal expression of many genes related glioma leads cell growth metastasis. Phospholipase C beta 1 (PLCB1) plays critical roles in intracellular transduction regulating signal activation, are important tumorigenesis. Therefore, it could bind miRNA as target gene. The purpose our study was confirm PLCB1 role suppressing progression. We found the miR-423-5p reduced, but increased, tissues cells. To explore whether affects PLCB1, bioinformatics approach suggested can directly PLCB1. Moreover, we observed, using luciferase reporter assays, 3'-UTR. Functionally, overexpression attenuate proliferation, invasion, migration promote apoptosis Furthermore, enhance p-ERK Taken together, deduced inhibited proliferation metastasis by targeting also promotes These results suggest targets 3'-UTR inhibit invasion through ERK-dependent pathway glioma, miR-423-5p/PLCB1 axis may be potential for new therapeutic strategies treat glioma.