MicroRNA-423-5p Promotes Autophagy in Cancer Cells and Is Increased in Serum From Hepatocarcinoma Patients Treated With Sorafenib.

摘要: Hepatocellular carcinoma (HCC) is the third cause of cancer-related deaths worldwide. Sorafenib only approved drug for patients with advanced HCC but has shown limited activity. microRNAs (miRs) have been involved in several neoplasms including suggesting their use or targeting as good tools treatment. The purpose this study was to identify novel approaches sensitize cells sorafenib through miRs. miR-423-5p validated positive regulator autophagy cell lines by transient transfection miR and anti-miR molecules. expression level evaluated real-time polymerase chain reaction (PCR) sera collected from 39 before after treatment sorafenib. were cotreated effects on cycle, apoptosis, evaluated. Secretory upregulated both vitro vivo its increase correlated response therapy since 75% which an secretory miR423-5p found partial remission stable disease 6 moths beginning therapy. transfected showed percentage S-phase cycle paralleled a similar autophagic at fluorescence activated sorter (FACS) transmission electron microscopy. Our results suggest can be used useful tool predict regulation cells.