Growth hormone secretagogue receptor family members and ligands.
作者： Roy G Smith , Reid Leonard , Alex R T Bailey , Oksana Palyha , Scott Feighner
关键词: Ghrelin 、 Growth hormone secretagogue receptor 、 Agonist 、 Genomic library 、 Genetics 、 Gene 、 Partial agonist 、 Genome 、 Receptor 、 Biology 、 Molecular biology
摘要: We have previously reported the cloning and characterization of a new orphan G-protein-coupled receptor (GPC-R), growth hormone secretagogue (GHS-R), shown that this mediates activity hormone-releasing peptides (GHRPs) nonpeptide ligands such as L-692,429 MK-0677. Because GHS-R obviously does not belong to any known GPC-R subfamilies, we searched for family members by screening human genomic library using low-stringency hybridization Pufferfish library. The was selected because its compact genome. From library, homolog, GPR 38, with 52% identity isolated. three were gene having 58% GHS-R, on expression in HEK293 cells, activated GHRP-6 These results indicate has been conserved at least 400 million years genome is appropriate isolation members. In our search endogenous receptors GPR38, showed adenosine partial agonist motilin ligand GPR38. also confirmed ghrelin full GHS-R.
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