Capillary isoelectric focusing-electrospray mass spectrometry for protein analysis
作者： Qing. Tang , A. Kamel. Harrata , Cheng S. Lee
DOI: 10.1021/AC00115A021
关键词: Isoelectric focusing 、 Electrospray ionization 、 Capillary electrophoresis 、 Mass spectrum 、 Analytical chemistry 、 Mass spectrometry 、 Electrospray 、 Chromatography 、 Myoglobin 、 Chemistry 、 Detection limit
摘要: On-line combination of capillary isoelectric focusing (CIEF) with electrospray mass spectrometry (ESMS) as a two-dimensional separation system is demonstrated. Mixtures model proteins including cytochrome c (horse heart), myoglobin and carbonic anhydrase II (bovine erythrocyte) are focused cathodically mobilized in polyacrylamide-coated capillary. At the end CIEF capillary, protein zones analyzed by coupled on-line to an interface coaxial sheath flow configuration. The effects carrier ampholyte concentration on ionization spectra presented discussed. In this study, effect permits analysis very dilute samples. A typical factor 50-100 times observed. detection limit for full-scan CIEF-ESMS range 10{sup -7} M, 2 orders magnitude over that possible normal zone electrophoresis ESMS. 35 refs., 5 figs., tabs.
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