Embryo-specific Proteins in Cyclamen persicum Analyzed with 2-D DIGE

摘要: Somatic embryogenesis can be used to produce artificial seeds of Cyclamen persicum, one the most important ornamental plants for European market, both as a potted plant in northern Europe and bedding cool winters southern Europe. The aim this study was obtain new insights into molecular biology somatic embryogenesis, which turn useful improvement tissue culture methodology. Total proteins were characterized from two isogenic cell lines that embryogenic never has shown any capacity. extracted separated by two-dimensional differential gel electrophoresis (2-D DIGE) selected treated using ETTAN Dalt Spot Handling Workstation. Protein identification performed MALDI-TOF-MS. More than 1200 detected; 943 common lines. different protein patterns non-embryogenic obvious: One hundred eight more abundant cells, 97 cells. Among differentially expressed proteins, 128 identified. MALDI-TOF-MS analysis enabled 27 spots proposed candidates embryo-specific they unique line. identified are involved variety cellular processes, including proliferation, processing, signal transduction, stress response, metabolism, energy state, but majority processing metabolism. main functions putative have been discussed proportion their role process.