From callus to embryo: a proteomic view on the development and maturation of somatic embryos in Cyclamen persicum

摘要: In this study, the proteome structures following pathway in somatic embryogenesis of Cyclamen persicum were analysed via high-resolution 2D-SDS-PAGE with two objectives: (1) to identify significant physiological processes during and (2) improve maturation embryos. Therefore, effects maturation-promoting plant growth regulator abscisic acid (ABA) high sucrose levels on torpedo-shaped embryos investigated. total, 108 proteins differential abundance identified using a combination tandem mass spectrometry digital reference map. callus, enzymes related energy supply especially distinct, most likely due demand caused by fast cell division. The switch from callus globular embryo as well was associated controlled proteolysis ubiquitin-26S proteasome pathway. Storage compound accumulation first detected 21 days after transfer (PGR)-free medium early Increase auxin-amidohydrolase suggests possible increase auxin release late stages Cyclamen. A development-specific isoelectric point catalases has been reported for time embryogenesis. Several represent markers different developmental analysed. High ABA treatment promoted storage compounds Additionally, primary metabolic pathways decreased proteomes ABA-treated Thus, concentration culture improved consequently quality C. persicum.