Genome analysis of quorum sensing Cedecea neteri SSMD04 leads to identification of its novel signaling synthase (cneI), cognate receptor (cneR) and an orphan receptor

摘要: Cedecea neteri is a very rare human pathogen. We have isolated strain of C. SSMD04 from pickled mackerel sashimi identified using molecular and phenotypics approaches. Using the biosensor Chromobacterium violaceum CV026, we demonstrated presence short chain N-acyl-homoserine lactone (AHL) type quorum sensing (QS) activity in SSMD04. Triple quadrupole LC/MS analysis revealed that produced N-butyryl-homoserine (C4-HSL). With available genome information SSMD04, went on to analyse pair luxI/R homologues this share highest similarity with croI/R Citrobacter rodentium. The AHL synthase, which named cneI(636 bp), was found sequences At distance 8bp cneI sequence encoding hypothetical protein, potentially cognate receptor, luxR homologue it as cneR. Analysis protein amino acid reveals two signature domains, autoinducer-binding domain C-terminal effector typical characteristic luxR. In addition, harboured an orphan most closely related easR Enterobacter asburiae. To our knowledge, first report production neteri, discovery its homologues, receptor whole sequence.