Online enzyme discrimination and determination of substrate enantiomers based on electrophoretically mediated microanalysis.
作者： Wenwen Zhao , Miaomiao Tian , Rongbin Nie , Yulin Wang , Liping Guo
DOI: 10.1021/AC301125J
关键词: Chemistry 、 Repeatability 、 Detection limit 、 Chromatography 、 Microanalysis 、 Enzyme catalysis 、 Nicotinamide adenine dinucleotide 、 Lactate dehydrogenase 、 Substrate (chemistry) 、 Enantiomer
摘要: We proposed the first application of an electrophoretically mediated microanalysis (EMMA) method for fast online discrimination and determination substrate enantiomers, which was achieved by just one EMMA assay. Lactate dehydrogenase (LDH)-catalyzed reaction studied to evaluate feasibility performance presented method. The l- d-LDH chiral enzymatic reactions, are highly stereoselective lactate were initiated successively in capillary, corresponding products, nicotinamide adenine dinucleotide (NADH), discriminated detected UV absorption. Excellent linear dependence two NADH peak intensities on concentration enantiomers obtained within a wide range 0.1–10 mM. limit detection 26 μM d-lactate 49 l-lactate (S/N = 3). Good repeatability demonstrated with relative standard deviation (RSD) < 6.3% height RSD...
acs.org
sci-hub.se 参考文章(32)
Miroslav Pohanka, Petr Zbořil, Amperometric Biosensor for D-Lactate Assay Food Technology and Biotechnology. ,vol. 46, pp. 107- 110 ,(2008)
H. Henry, N. Marmy Conus, P. Steenhout, A. Béguin, O. Boulat, Sensitive determination of d-lactic acid and l-lactic acid in urine by high-performance liquid chromatography-tandem mass spectrometry Biomedical Chromatography. ,vol. 26, pp. 425- 428 ,(2012) , 10.1002/BMC.1681
Zdeněk Glatz, Determination of enzymatic activity by capillary electrophoresis Journal of Chromatography B. ,vol. 841, pp. 23- 37 ,(2006) , 10.1016/J.JCHROMB.2006.02.051
Fira Shapiro, Nissim Silanikove, Rapid and accurate determination of d- and l-lactate, lactose and galactose by enzymatic reactions coupled to formation of a fluorochromophore: Applications in food quality control Food Chemistry. ,vol. 119, pp. 829- 833 ,(2010) , 10.1016/J.FOODCHEM.2009.07.029
Shuji Kodama, Atsushi Yamamoto, Akinobu Matsunaga, Direct chiral resolution of aliphatic α-hydroxy acids using 2-hydroxypropyl-β-cyclodextrin in capillary electrophoresis Analyst. ,vol. 124, pp. 55- 59 ,(1999) , 10.1039/A807351A
Yi Fan, Gerhard K. E. Scriba, Electrophoretically mediated microanalysis assay for sirtuin enzymes ELECTROPHORESIS. ,vol. 31, pp. 3874- 3880 ,(2010) , 10.1002/ELPS.201000336
Cristina IC Silvestre, Paula CAG Pinto, Marcela A Segundo, M Lúcia MFS Saraiva, José LFC Lima, None, Enzyme based assays in a sequential injection format: a review. Analytica Chimica Acta. ,vol. 689, pp. 160- 177 ,(2011) , 10.1016/J.ACA.2011.01.048
Yuanfang Chen, Liangliang Xu, Wenwen Zhao, Liping Guo, Li Yang, Method for the sequential online analysis of enzyme reactions based on capillary electrophoresis. Analytical Chemistry. ,vol. 84, pp. 2961- 2967 ,(2012) , 10.1021/AC3001644
Fira Shapiro, Nissim Silanikove, Rapid and accurate determination of malate, citrate, pyruvate and oxaloacetate by enzymatic reactions coupled to formation of a fluorochromophore: Application in colorful juices and fermentable food (yogurt, wine) analysis. Food Chemistry. ,vol. 129, pp. 608- 613 ,(2011) , 10.1016/J.FOODCHEM.2011.04.074
Li Yang, Chunyang Chen, Yuanfang Chen, Jing Shi, Sidong Liu, Liping Guo, Haifeng Xu, High temporal resolution monitoring of enzyme reaction and inhibition using optically gated vacancy capillary electrophoresis and immobilized enzyme. Analytica Chimica Acta. ,vol. 683, pp. 136- 142 ,(2010) , 10.1016/J.ACA.2010.10.013