EDTA inhibits in vitro substrate adherence capacity of macrophages: Endodontic implications
作者： J.J. Segura , J.R. Calvo , J.M. Guerrero , A. Jimenez-Planas , C. Sampedro
DOI: 10.1016/S0099-2399(97)80046-5
关键词: In vitro 、 Immunology 、 Root canal 、 Materials science 、 Incubation 、 Pharmacology 、 Chelation 、 Ethylenediamine tetraacetate 、 Endodontics 、 Calcium 、 General Dentistry
摘要: The disodium salt of ethylenediamine tetraacetate (EDTA) is a calcium ion chelatOR used in endodontics to enlarge root canals. This study investigated the effect EDTA on substrate adherence capacity rat inflammatory macrophages determine if leakage periapical tissues during canal therapy can alter macrophage function. Inflammatory were obtained from Wistar rats and resuspended RPMI-1640 medium. Substrate assays carried out Eppendorf tubes fOR 15 min incubation at 37°C humidified atmosphere 5% CO 2 . index (Al) was calculated. Results showed that decreased time dose-dependent manner. lowest concentration caused significant inhibition Al 50 mM (p ) 194 ± 20
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