The role of ethylenediamine tetraacetic acid (EDTA) as in vitro anticoagulant for diagnostic purposes

摘要: Anticoagulants are used to prevent clot formation both in vitro and vivo. In the specific field of diagnostics, anticoagulants commonly added collection tubes either maintain blood fluid state for hematological testing or obtain suitable plasma coagulation clinical chemistry analyses. Unfortunately, no universal anticoagulant that could be evaluation several laboratory parameters a sample from single test tube is available so far. Ethylenediamine tetraacetic acid (EDTA) polyprotic containing four carboxylic groups two amine with lone-pair electrons chelate calcium other metal ions. Calcium necessary wide range enzyme reactions cascade its removal irreversibly prevents clotting within tube. Historically, EDTA has been recommended as choice because it allows best preservation cellular components morphology cells. The remarkable expansion volume complexity over recent decades amplified potential spectrum applications this anticoagulant, which can stabilize variety traditional innovative tests. Specific data on behavior an hematology, including possible pitfalls, presented. use measuring cytokines, protein peptides, cardiac markers described, outline protection labile molecules provided by anticoagulant. proteomics general also described comparison serum samples. Finally, uses alternative instead illustrated.