BRACHYURY and CDX2 Mediate BMP-Induced Differentiation of Human and Mouse Pluripotent Stem Cells into Embryonic and Extraembryonic Lineages
作者： Andreia S. Bernardo , Tiago Faial , Lucy Gardner , Kathy K. Niakan , Daniel Ortmann
DOI: 10.1016/J.STEM.2011.06.015
关键词: Endoderm 、 Induced pluripotent stem cell 、 Brachyury 、 NODAL 、 Embryonic stem cell 、 Mesoderm 、 Trophoblast 、 Cellular differentiation 、 Molecular biology 、 Cell biology 、 Biology
摘要: BMP is thought to induce hESC differentiation toward multiple lineages including mesoderm and trophoblast. The BMP-induced trophoblast phenotype a long-standing paradox in stem cell biology. Here we readdressed function hESCs mouse epiblast-derived cells. We found that BMP4 cooperates with FGF2 (via ERK) inhibit endoderm differentiation. These conditions induced cells high levels of BRACHYURY (BRA) coexpressed CDX2. BRA was necessary for preceded CDX2 expression; both genes were essential expression not only mesodermal but also trophoblast-associated genes. Maximal the latter seen absence FGF these moreover they differed surface epigenetic properties from placental conclude induces human pluripotent primarily form mesoderm, rather than trophoblast, acting through
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