Physiological responses of Orthosiphon stamineus plantles to gamma irradiation.
作者： Abdul Rahim Harun , A. G. Lai , Sobri Hussein , Kiong LingPick
DOI:
关键词: Shoot 、 Enzyme assay 、 Irradiation 、 Orthosiphon stamineus 、 Food science 、 Explant culture 、 Chlorophyll 、 Biochemistry 、 Peroxidase 、 Rosmarinic acid 、 Biology
摘要: In vitro mutagenesis by means of gamma irradiation can be used to produce economically improved mutants. Gamma was induce biochemical changes in a prominent medicinal plant, Orthosiphon stamineus. Shoot tip explants O. stamineus were subjected at 0, 10, 20, 30, 40, 50, 60 and 70 Gray (Gy). Biochemical studies on revealed that the total soluble protein chlorophyll content decreased notably as dosage increases. However, plantlets irradiated 10 20Gy exhibited 39.61 ± 0.66mg/g FW 34.00 0.85mg/g FW, respectively which higher than all other treatments. Severe increased specific activity peroxidase observed especially high dosages (70Gy). Analysis banding profile using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis (SDS-PAGE) demonstrated treatment did not significant change constituent. Nine major bands relatively similar intensities 12, 15, 16, 25, 27, 46, 80kDa. Rosmarinic acid lowest (5.27±0.35mg/g FW) 10Gy, highest (8.40±0.57mg/g 30Gy. it suggested low an apparent rosmarinic irregular trend production observed. Due potential future pharmaceutical industries, further research this plant could done facilitate propagation superior mutants is able vast quantities valuable secondary metabolites.
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