Insulin receptor tyrosine kinase activity and substrate 1 (IRS-1) expression in human myometrium and leiomyoma
作者： Rafael B Orcy , Ilma Brum , Roselis SM da Silva , Luiz CR Kucharski , Helena v E Corleta
DOI: 10.1016/J.EJOGRB.2005.02.025
关键词: Myometrium 、 Biology 、 Insulin receptor 、 Uterine leiomyoma 、 Receptor tyrosine kinase 、 Internal medicine 、 Endocrinology 、 Insulin 、 Tyrosine kinase 、 IRS1 、 Leiomyoma 、 Obstetrics and gynaecology 、 Reproductive medicine
摘要: Abstract Background Uterine leiomyomas are the commonest tumors of genital tract. Growth factors seem to be implicated in development leiomyoma. Objective To determine insulin receptor (IR) tyrosine kinase activity – phosphorylation exogenous substrate poly(Glu 4: Tyr 1) and 1 expression normal myometrium Study design The study group consisted 12 women with leiomyoma undergoing routine hysterectomy. Samples adjacent were obtained at time operation. Plasma membrane fractions prepared samples incubated without 1). IRS-1 was studied whole lysate via Western blotting using specific antibodies. Data analyzed Student's t -test. Results (1.566 ± 0.177) (1.98 ± 0.612) similar ( P = 0.774). levels (0.190 ± 0.022) (0.226 ± 0.022) not different = 0.184). Conclusions There no difference IR (phosphorylation substrate) between leiomyomata. Other steps signaling cascade require further investigate role
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