Heterologous production of Rhizopus oryzae lipase in Pichia pastoris using the alcohol oxidase and formaldehyde dehydrogenase promoters in batch and fed-batch cultures
作者： Oriol Cos , David Resina , Pau Ferrer , José Luis Montesinos , Francisco Valero
DOI: 10.1016/J.BEJ.2005.04.005
关键词: Pichia pastoris 、 Lipase 、 Formaldehyde dehydrogenase 、 Promoter 、 Alcohol oxidase 、 Heterologous 、 Triacylglycerol lipase 、 Rhizopus oryzae 、 Biology 、 Biochemistry
摘要: Abstract A Rhizopus oryzae lipase (ROL) has been expressed in Pichia pastoris as a reporter gene using two different regulated promoters. Both phenotypes, Muts and Mut+, have used for the expression under control of alcohol oxidase 1 promoter (PAOX1). Moreover, new formaldehyde dehydrogenase (PFLD1) tested. PFLD1 allows design methanol-free culture strategies, being methylamine less volatile flammable inducer. The comparison batch fed-batch cultures promoters shown that (PAOX1) is somewhat more efficient than Mut+ systems, with 1.1- 1.4-fold higher specific productivity (U lipase/g biomass h), respectively. However, all three systems was very similar (2500 U lipase/l h). These results demonstrate heterologous proteins transcriptional promising alternative to well-known PAOX1.
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