Neuro-glial cannabinoid receptors modulate signaling in the embryonic avian retina

摘要: Abstract Endocannabinoids are endogenous lipids that activate selective G protein coupled receptors (CB1 and CB2), mostly found at neuronal presynaptic sites in the nervous system. One of main consequences activation CB is a decrease GABA or glutamate release, controlling cell excitability. Here we studied expression CB1 CB2 E8C8 cultured retina cells (embryonic day 8 days in vitro) using immunocytochemistry western blot analysis. We also evaluated their functions terms cyclic AMP (cAMP) production, single calcium imaging (SCCI) release induced basal conditions activated by l -Aspartate (L-ASP) cultures under ischemia young chick retina. show both cannabinoid expressed retinal neurons glial cells. WIN 55,212-2 (WIN, CB1/CB2 agonist) decreased cAMP production avian embryonic conditions. led to number increased Ca2+ levels evoked ATP, but had no effect shifts KCl. Finally, inhibited [3H]-GABA KCl L-ASP, accumulated amacrine cells, amount released an oxygen glucose deprivation (OGD) condition. Altogether, our data indicate function as regulators signaling critical stages during synapse formation.