Studies of Carbohydrate-Binding Proteins from Enterotoxigenic Escherichia coli
作者： Lena Jansson
DOI:
关键词: Microbiology 、 Heat-stable enterotoxin 、 Enterotoxigenic Escherichia coli 、 Bacteria 、 Escherichia coli 、 Chemistry 、 Heat-labile enterotoxin 、 Glycoconjugate 、 Ligand binding assay 、 Virulence
摘要: 3 POPULARVETENSKAPLIG SAMMANFATTNING 4 PAPERS INCLUDED IN THIS THESIS 5 CONTENTS 6 ABBREVIATIONS 7 BACKGROUND 8 INTRODUCTION MICROBIAL ADHESION CARBOHYDRATES 9 GLYCOCONJUGATES ON THE MAMMALIAN CELL SURFACE GLYCOSPHINGOLIPIDS 10 PROTEIN-CARBOHYDRATE INTERACTIONS 13 ESCHERICHIA COLI Enterotoxigenic E. coli VIRULENCE FACTORS OF ETEC 14 I. Colonization factors CFA/I 15 CS6 II. Enterotoxins THERAPEUTIC STRATEGIES 17 METHODOLOGY 18 ISOLATION EXPRESSION AND PURIFICATION B-SUBUNITS THIN-LAYER CHROMATOGRAPHY 19 TLC BINDING ASSAY 20 INHIBITION MICROTITER WELL CULTURE LABELING BACTERIA RADIOLABELING 21 AIMS 22 RESULTS DISCUSSION 23 PAPER I II 26 III 29 IV 31 CONCLUDING REMARKS 34 ACKNOWLEDGEMENTS 36 REFERENCES 37 APPENDIX 43
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