Cross-presentation of HCMV chimeric protein enables generation and measurement of polyclonal T cells.

摘要: CD8+ T cell immunity has a critical function in controlling human cytomegalovirus (HCMV) infection. In immunocompromized individuals, HCMV reactivation or disease can lead to increased morbidity and mortality, particularly transplant recipients. this setting, adoptive transfer of HCMV-specific cells is promising vaccine strategy restore viral immunity, with most clinical approaches focussing on the use peptides for generation single epitope-specific cells. We show that using an IE1-pp65 chimeric protein as antigen source promotes effective cross-presentation, by monocyte-derived dendritic (MoDCs), generate polyclonal epitopes. By exploring leukocyte (HLA)-restricted immunodominance hierarchies both within across two immunodominant proteins, we HLA-B7 epitopes elicit higher responses compared HLA-A1, -A2 -B8. This study provides important evidence highlighting efficacy importance designing future therapeutic vaccines.