A Direct Biocombinatorial Strategy toward Next Generation, Mussel-Glue Inspired Saltwater Adhesives

摘要: Biological materials exhibit remarkable, purpose-adapted properties that provide a source of inspiration for designing new to meet the requirements future applications. For instance, marine mussels are able attach broad spectrum hard surfaces under hostile conditions. Controlling wet-adhesion synthetic macromolecules by analogue processes promises strongly impact sciences offering advanced coatings, adhesives, and glues. The de novo design mimic complex aspects mussel adhesion still constitutes challenge. Phage display allows material scientists specifically interacting molecules with tailored affinity surfaces. Here, we report on integration enzymatic processing steps into phage biopanning expand biocombinatorial procedure enable direct selection enzymatically activable peptide domains. Adsorption isotherms single molecule force spectroscopy show those peptides bioadhesion, such as activation (by tyrosinase), switchability from weak strong binders, adsorption saltwater Furthermore, peptide-poly(ethylene oxide) conjugates synthesized generate protective which possess anti-fouling suppress irreversible interactions blood-plasma protein cocktails. extended provides generic way non-natural domains, not only nature but also improve biological sequence sections extractable mussel-glue proteins. manage combine several tasks in minimal 12-mer thus pave overcome major challenges technical wet