A cell surface chondroitin sulfate proteoglycan, immunologically related to CD44, is involved in type I collagen-mediated melanoma cell motility and invasion.

摘要: The metastatic spread of tumor cells occurs through a complex series events, one which involves the adhesion to extracellular matrix (ECM) components. Multiple interactions between cell surface receptors an adherent and surrounding ECM contribute motility invasion. current studies evaluate role chondroitin sulfate proteoglycan (CSPG) in adhesion, motility, invasive behavior highly mouse melanoma line (K1735 M4) on type I collagen matrices. By blocking production CSPG with p-nitrophenyl beta-D-xylopyranoside (beta-D-xyloside), compound that uncouples from core protein synthesis, we observed corresponding decrease into gels. Melanoma could also be inhibited by removing chondroitinase. In contrast, collagen-mediated spreading were not affected either beta-D-xyloside or chondroitinase treatments. These results suggest is primary receptor, but may play invasion at level cellular translocation. Furthermore, purified was shown, affinity chromatography solid phase binding assays, bind this interaction shown mediated, least part, sulfate. Additionally have determined composed 110-kD recognized anti-CD44 antibodies Western blots. Collectively, our data suggests CD44-related important invasion, portion seems critical component mediating effect.