In vitro study of vancomycin release and osteoblast-like cell growth on structured calcium phosphate-collagen.
作者： Weeraphat Pon-On , Narattaphol Charoenphandhu , Jarinthorn Teerapornpuntakit , Jirawan Thongbunchoo , Nateetip Krishnamra
DOI: 10.1016/J.MSEC.2012.12.046
关键词: Cell adhesion 、 Calcium 、 Biophysics 、 Nanotechnology 、 Simulated body fluid 、 In vitro 、 Materials science 、 Substrate (chemistry) 、 Biocompatibility 、 Sonication 、 Drug delivery
摘要: Abstract A drug delivery vehicle consisting of spherical calcium phosphate-collagen particles covered by flower-like (SFCaPCol) blossoms composed nanorod building blocks and their cellular response is studied. The structure was achieved a combination sonication freeze-drying. SFCaPCol have high surface area approximately 280 m 2 g − 1 . blossom-like formation having allows loading efficiency 77.82%. release profile for one drug, vancomycin (VCM), shows long term sustained in simulated body fluid (SBF), phosphate buffer saline (PBS, pH 7.4) solution culture media over 2 weeks with cumulative ~ 53%, 75% 50%, respectively, the first 7 days. biocompatibility VCM-loaded scaffold determined vitro cell adhesion proliferation tests rat osteoblast-like UMR-106 cells. MTT indicated that cells were viable after exposure to VCM loaded SFCaPCol, meaning (the blossoms) did not impair cell's viability. density on substrate seen increase increasing cultured time.
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