Recellularization of Well-Preserved Acellular Kidney Scaffold Using Embryonic Stem Cells

摘要: For chronic kidney diseases, there is little chance that the vast majority of world's population will have access to renal replacement therapy with dialysis or transplantation. Tissue engineering would help address this shortcoming by regeneration damaged using naturally occurring scaffolds seeded precursor cells. The aims present study were optimize production three-dimensional (3D) rat whole-kidney shortening duration organ decellularization process detergents avoid nonionic compounds, investigate integrity extracellular matrix (ECM) structure and enhance efficacy scaffold cellularization physiological perfusion method. Intact kidneys successfully decellularized after 17 h sodium dodecyl sulfate. preserved 3D architecture blood vessels, glomeruli, tubuli as shown transmission scanning electron microscopy. Micro-computerized tomography (micro-CT) scan confirmed integrity, patency, connection vascular network. Collagen IV, laminin, fibronectin staining similar those native tissues. After infusion murine embryonic stem (mES) cells through artery, pressure-controlled recirculating cell medium for 24 72 h, almost completely retained into uniformly distributed in network glomerular capillaries without major signs apoptosis. Occasionally, mES reached peritubular capillary tubular compartment. We observed loss pluripotency start differentiation toward meso-endodermal lineage. Our findings indicate that, proposed optimized protocol, can be efficiently produce ECM a relatively short time, rapid recellularization structures glomeruli. This experimental setup may open possibility obtain long lasting vitro perfusion.