Exopolysaccharide assay in Escherichia coli microcolonies using a cleavable fusion protein of GFP-labeled carbohydrate-binding module.
作者： Yoshihiro Ojima , Asep Suparman , Minh Hong Nguyen , Makiko Sakka , Kazuo Sakka
DOI: 10.1016/J.MIMET.2015.05.013
关键词: Biochemistry 、 Carbohydrate-binding module 、 Green fluorescent protein 、 Molecular biology 、 Protease 、 Fusion protein 、 Escherichia coli 、 Fluorescence 、 Cleavage (embryo) 、 Biology
摘要: A fused protein composed of a carbohydrate-binding module and green fluorescence (GFP) was developed to measure the exopolysaccharides (EPShs) present in Escherichia coli microcolonies. The cleavage GFP part this using site-specific protease allowed for non-invasive quantitative evaluation EPShs.
elsevier.com
sci-hub.se 参考文章(13)
Martin Strathmann, Jost Wingender, Hans-Curt Flemming, Application of fluorescently labelled lectins for the visualization and biochemical characterization of polysaccharides in biofilms of Pseudomonas aeruginosa. Journal of Microbiological Methods. ,vol. 50, pp. 237- 248 ,(2002) , 10.1016/S0167-7012(02)00032-5
X. Chen, P. S. Stewart, Role of electrostatic interactions in cohesion of bacterial biofilms. Applied Microbiology and Biotechnology. ,vol. 59, pp. 718- 720 ,(2002) , 10.1007/S00253-002-1044-2
Yu Liu, Shu-Fang Yang, Yong Li, Hui Xu, Lei Qin, Joo-Hwa Tay, The influence of cell and substratum surface hydrophobicities on microbial attachment. Journal of Biotechnology. ,vol. 110, pp. 251- 256 ,(2004) , 10.1016/J.JBIOTEC.2004.02.012
Virginia L. Thomas, Barbara A. Sanford, Rita Moreno, Mary A. Ramsay, Enzyme-linked lectinsorbent assay measures N-acetyl-D-glucosamine in matrix of biofilm produced by Staphylococcus epidermidis. Current Microbiology. ,vol. 35, pp. 249- 254 ,(1997) , 10.1007/S002849900248
V. Leriche, P. Sibille, B. Carpentier, Use of an enzyme-linked lectinsorbent assay to monitor the shift in polysaccharide composition in bacterial biofilms. Applied and Environmental Microbiology. ,vol. 66, pp. 1851- 1856 ,(2000) , 10.1128/AEM.66.5.1851-1856.2000
Tatsuya Masuko, Akio Minami, Norimasa Iwasaki, Tokifumi Majima, Shin-Ichiro Nishimura, Yuan C. Lee, Carbohydrate analysis by a phenol-sulfuric acid method in microplate format. Analytical Biochemistry. ,vol. 339, pp. 69- 72 ,(2005) , 10.1016/J.AB.2004.12.001
E. Burton, N. Yakandawala, K. LoVetri, M. S. Madhyastha, A microplate spectrofluorometric assay for bacterial biofilms Journal of Industrial Microbiology & Biotechnology. ,vol. 34, pp. 1- 4 ,(2006) , 10.1007/S10295-006-0086-3
AWST Jahn, PH Nielsen, Extraction of extracellular polymeric substances (EPS) from biofilms using a cation exchange resin Water Science and Technology. ,vol. 32, pp. 157- 164 ,(1995) , 10.1016/0273-1223(96)00020-0
David Davies, Understanding biofilm resistance to antibacterial agents. Nature Reviews Drug Discovery. ,vol. 2, pp. 114- 122 ,(2003) , 10.1038/NRD1008
Minh Hong Nguyen, Yoshihiro Ojima, Makiko Sakka, Kazuo Sakka, Masahito Taya, Probing of exopolysaccharides with green fluorescence protein-labeled carbohydrate-binding module in Escherichia coli biofilms and flocs induced by bcsB overexpression Journal of Bioscience and Bioengineering. ,vol. 118, pp. 400- 405 ,(2014) , 10.1016/J.JBIOSC.2014.03.005