Complementation of a red-light-indifferent cyanobacterial mutant.

作者: G. G. Chiang , M. R. Schaefer , A. R. Grossman

DOI: 10.1073/PNAS.89.20.9415

关键词: Transposable elementGenomic libraryComplementationBiologyMutantSouthern blotNucleic acid sequencegenomic DNAGeneGenetics

摘要: Many cyanobacteria alter their phycobilisome composition in response to changes light wavelength a process termed complementary chromatic adaptation. Mutant strains FdR1 and FdR2 of the filamentous cyanobacterium Fremyella diplosiphon are characterized by aberrant Instead adjusting different wavelengths light, behave as if they always green light; do not respond red light. We have previously reported complementation conjugal transfer wild-type genomic library. The complementing DNA has now been localized genetic analysis region on rescued subclone that contains gene designated rcaC. This is also able complement FdR2. Southern blot from indicates these harbor insertions within rcaC sequence may resulted activity transposable elements. predicted amino acid RcaC shares strong identity regulators bacterial two-component regulatory systems. relationship discussed context signal-transduction pathway mediating regulation genes encoding polypeptides during

参考文章(2)
B. A. Whitton, N. G. Carr, The biology of cyanobacteria. University of California Press. ,(1982)
Tom Maniatis, Joseph Sambrook, E. F. Fritsch, Molecular Cloning: A Laboratory Manual ,(2001)