Neuropeptide Y in central control of feeding and interactions with orexin and leptin.

摘要: Neuropeptide (NPY) increases feeding when injected into the brain. In this study, we tested hypothesis that its action might be related to regulation of orexin and leptin systems in rats. Intracerebroventricular administration NPY (1 nmol/5 µL) stimulated Injection an antibody orexin-A inhibited feeding, suggesting endogenous exerts a stimulatory tone on feeding. injection antiserum before significantly attenuated response NPY. On other hand, ip pretreatment with (2 mg/kg) decreased food intake NPY-induced We then examined whether orexin-containing neurons are activated under stimulation intracerebroventricular or suppression leptin, using Fos-like immunoreactivity (FLI) as marker neural activation. observed FLI was induced paraventricular, supraoptic, dorsomedial nuclei well lateral hypothalamic area (LHA) following Double staining anti-Fos antiorexin antibodies revealed 23.4% LHA expressed after injection. Approximately 7.8% orexin-positive coexpressed Fos plus Our data indicate functional interaction among NPY, orexin, exists may contribute central appetite.