Immunocytochemical localization of the 27K β-crystallin polypeptide in the mouse lens during development using a specific monoclonal antibody: Implications for cataract formation in the Philly mouse

摘要: The Philly mouse develops a hereditary cataract about 5 weeks after birth. Although the causative agent is not known, data suggest that there correlation between formation and selective absence of 27 kilodalton (27K) beta-crystallin lens polypeptide. ontogeny 27K polypeptide was examined in normal mice order to evaluate its role development determine what impact may have on lens. A monoclonal antibody used with PAP method immunocytochemically localize lenses during development. beta-Crystallins detected polyclonal antisera were found differentiated fiber cells throughout In contrast, specific inner part (nucleus), but specifically localized outer called cortex. only elongating mitotically active epithelial cells. minor component 2-day-old lens, comprised large portion 16-day-old including anterior posterior poles. These show embryonic becomes major contributor postnatal abundant contribute observed failure differentiate birth or be deleterious some other manner polypeptide, defect which precedes mouse, intriguing since it suggests relationship this clarity.