A comparison of the microbiome and the metabolome of different regions of the equine hindgut

摘要: The microbiome and associated metabolome of faecal samples were compared to those from the caecum right dorsal colon horses ponies euthanised for nonresearch purposes by investigating microbial population community structure as well their functional metabolic products. Through use 16S rRNA gene dendrograms, was shown cluster separately other gut regions. gene-based quantitative PCR (q-PCR) also demonstrated differences between Metabolites identified Fourier transform infrared clustered in a similar way specific products (volatile fatty acids ammonia) varied region. Protozoal 18S rDNA concentration archaeal mcrA quantified q-PCR found higher numbers than Diversity calculations using Simpson Shannon–Wiener indices diversity faeces caecum. All findings this study suggest that are likely represent some extent but not caecum, indicating careful consideration is required when planning investigations hindgut horse.