Multi-walled carbon nanotubes suppress potassium channel activities in PC12 cells
作者： Haifei Xu , Juan Bai , Jie Meng , Wei Hao , Haiyan Xu
DOI: 10.1088/0957-4484/20/28/285102
关键词: Nanomaterials 、 Potassium 、 Membrane potential 、 Materials science 、 Oxidative stress 、 Intracellular free calcium 、 Potassium channel 、 Biophysics 、 Carbon nanotube 、 Biochemistry 、 Reactive oxygen species
摘要: The advancement in nanotechnology has produced technological and conceptual breakthroughs but the effects nanomaterials have on organisms at cellular level are poorly understood. Here we report that carboxyl-terminated multi-walled carbon nanotubes (MWCNTs) act as antagonists of three types potassium channels assessed by whole-cell patch clamp electrophysiology undifferentiated pheochromocytoma (PC12) cells. Our results showed MWCNTs suppress current densities Ito, IK IK1 a time-dependent irreversible manner. suppressions were most distinct 24 h after incubation with MWCNTs. However, did not significantly change expression levels reactive oxygen species (ROS) or intracellular free calcium also alter mitochondrial membrane potential (ΔΨm) PC12 These suggest oxidative stress was involved suppression densities. Nonetheless, currents will impact electrical signaling excitable cells such neurons muscles.
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