Vitellogenin Induction in Cyprinus carpio Treated with 17 β-Estradiol and 4-Nonylphenol
作者： Silvia Casini , M. Cristina Fossi , Gabriele Mori , Anne Bjornstad
关键词: Toxicity 、 Vitellogenin 、 Endocrine disruptor 、 Cyprinidae 、 Biology 、 Carp 、 Common carp 、 Nonylphenol 、 Cyprinus 、 Internal medicine 、 Endocrinology
摘要: The aim of the present study was to demonstrate induction ofvitellogenin in common carp (Cyprinus carpio) as abiomarker for monitoring freshwater ecosystems. Sexually undifferentiated specimens were treated experimentally with 17β-estradiol and increasing doses 4-nonylphenol levels plasma Vtg measured orderto: 1) validate an ELISA assay carpusing polyclonal rabbit anti-salmon antibody AA1 (Biosense, Norway); 2) check sensitivity juvenilesin producing response estrogen stimulation. grouptreated showed high (156%)with respect controls, also groups 4-nonylphenolshowed Vtg. group 100 mg kg-1b.wt 61%. A statistically significantcorrelation found between dose response. This preliminary a using AA1. With further evidence tested biomarker might be proposed large scale estrogenic effects caused by pollution urban industrial effluents.
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