Vitellogenin, a marker of estrogen mimicking contaminants in fishes: Characterization, quantification and interference by anti-estrogens

摘要: Vitellogenin (Vg), the estrogen inducible protein precursor to egg yolk, serves as an indicator of exposure mimicking environmental contaminants. Vg was isolated by size exclusion and ion exchange chromatography from plasma California halibut (Paralichthys californicus) treated with estrogen. MALDI TOF mass spectrometry (MS) analysis resulted in a molecular 188 kDa. MS/MS de novo sequencing provided evidence that has more than one form Vg. Similar on white sturgeon (Acipenser transmontamus) did not reveal adequate suggest The potential using other MS methods understand structure function are discussed. An ELISA for measurement optimized validated commercially available antibody developed another flatfish species, turbot. Inclusion overnight preincubation critical low detection limits. Increasing amount Tween-20 0.05% buffers most effective improving recoveries spiked samples. At IC50, average recovery samples 104% interplate CV 12%. working range assay 33-1000 ng/mL, while limit sample is 2.2 µg/mL. response model compounds 17beta-estradiol and/j-nonylphenol show this suitable further studies White native Sacramento River subject agricultural, municipal industrial waste water effluents likely contain different classes endocrine-disrupting Reductions 17beta-estradiol-induced vitellogenin levels were observed co-injected beta-naphthoflavone (BNF, 50 mg/kg), Ah receptor agonist. inhibition maximal when injected simultaneously versus prior treatment offish BNF. This timing effect compared increases ethoxyresorufin-O-deethylase (EROD) activity suggests directly due enhanced metabolism receptor-induced enzymes. Results study will be relevant those monitoring programs who measure vitellogenin, it important how (dioxin) active contaminants can influence endpoint.