MicroRNA-650 Was a Prognostic Factor in Human Lung Adenocarcinoma and Confers the Docetaxel Chemoresistance of Lung Adenocarcinoma Cells via Regulating Bcl-2/Bax Expression
作者： Jia-Yuan Huang , Shi-Yun Cui , Yi-Tian Chen , Hai-Zhu Song , Gui-Chun Huang
DOI: 10.1371/JOURNAL.PONE.0072615
关键词: Bcl-2-associated X protein 、 Chemotherapy regimen 、 Metastasis 、 Docetaxel 、 Cancer research 、 Gene silencing 、 Chemotherapy 、 microRNA 、 Biology 、 Pathology 、 Adenocarcinoma 、 General Biochemistry, Genetics and Molecular Biology 、 General Agricultural and Biological Sciences 、 General Medicine
摘要: Increasing evidence shows that dysregulation of microRNAs (miRNAs) is involved in malignant transformation. We investigated the clinical significance miR-650 and its involvement chemoresistance to docetaxel. Our results showed relative expression level was significantly higher LAD tissues than corresponding nontumor high found be associated with incidence lymph node metastasis, advanced stage poor prognosis patients. Univariate multivariate analyses indicated an independent prognostic factor for survival. Also, we correlated response patients docetaxel-based chemotherapy. Silencing could increase vitro sensitivity docetaxel-resistant cells docetaxel, while upregulation decreased parental docetaxel both vivo. Additionally, silencing enhance caspase-3-dependent apoptosis, which might ratio Bcl-2/Bax. Further researches suggested inhibitor growth 4 (ING4) a direct target miR-650. Downregulated or upregulated ING4 partially rescue effects mimics cells. Furthermore, docetaxel-responding tissues, inversely Thus, novel marker potential indicator chemosensitivity chemotherapy regimen.
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