Apolipoprotein-E Controls Adenosine Triphosphate-Binding Cassette Transporters ABCB1 and ABCC1 on Cerebral Microvessels After Methamphetamine Intoxication

摘要: Background and Purpose—Methamphetamine is a powerful addictive, which has been associated with ischemic stroke brain hemorrhage in humans. Whether how methamphetamine influences the expression of tight junctions adenosine triphosphate-binding cassette transporters, have previously shown to be regulated by apolipoprotein-E (ApoE) under conditions ischemia, was unknown. Methods—C57BL/6J mice received intraperitoneal injections (3 times 4 mg/kg separated 3 hours) either alone or combination ApoE receptor-2 inhibitor receptor-associated protein (40 μg/kg) inducible nitric oxide synthase 1400W (5 mg/kg). Animals were euthanized 24 hours after exposure. Tissue responses evaluated Western blots, immunoprecipitation, immunohistochemistry using total cerebral microvessel extracts. Results—Methamphetamine induced transient activation stress kinases c-Jun N-terminal kinase 1/2 p38 ...