Dynamics of Cytokinins in Apical Shoot Meristems of a Day-Neutral Tobacco during Floral Transition and Flower Formation
作者： Walter Dewitte , Adriana Chiappetta , Abdelkrim Azmi , Erwin Witters , Miroslav Strnad
DOI: 10.1104/PP.119.1.111
关键词: Shoot 、 Organogenesis 、 Flower formation 、 Isopentenyladenosine 、 Cytokinin 、 Botany 、 Bud 、 Biology 、 Zeatin 、 Nicotiana tabacum
摘要: This study considered cytokinin distribution in tobacco (Nicotiana tabacum L.) shoot apices distinct phases of development using immunocytochemistry and quantitative tandem mass spectrometry. In contrast to vegetative flower buds, we detected no free bases (zeatin, dihydrozeatin, or isopentenyladenine) prefloral transition apices. We also observed a 3-fold decrease the content ribosides (zeatin riboside, dihydrozeatin isopentenyladenosine) during this phase. The group concluded that organ formation (e.g. leaves flowers) is characterized by enhanced content, very low endogenous levels found apices, which showed organogenesis. immunocytochemical analyses revealed differing intracellular localization bases. Dihydrozeatin isopentenyladenine were mainly cytoplasmic perinuclear, whereas zeatin clear-cut nuclear labeling. To our knowledge, first time phenomenon has been reported. Cytokinins do not seem act as positive effectors phase Furthermore, differences at cellular level may be indicative specific physiological role processes.
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