Factors affecting in vitro shoot formation from vegetative shoot apices of apple and relationship between organogenic response and cytokinin localisation
作者： Simone D'Angeli , Paola Lauri , Walter Dewitte , Henry Van Onckelen , Emilia Caboni
DOI: 10.1080/11263500112331350700
关键词: Basal shoot 、 Callus 、 Cytokinin 、 Botany 、 Shoot 、 Malus 、 Zeatin 、 Auxin 、 Explant culture 、 Biology
摘要: ABSTRACT The effects of macro- and micro-elements, benzyladenine (BA) concentration, the period auxin application on adventitious shoot formation from callus originating vegetative apices were tested apple (Malus domestica Borkh) rootstock Jork 9. putative relationship between organogenic response cytokinin localisation was also studied by an immunolocalisation technique for in situ determination free cytokinins. use MS (Murashige & Skoog, 1962) salts medium instead those LP (Quoirin Lepoivre, 1977) had a strong positive effect both rate number shoots produced. highest induced using 17.8 μM BA presence 2.7 NAA maintaining explants 20 days darkness, then transferring them to fresh auxin-free light. studies, performed antibodies with marked specificity against zeatin isopentenyladenine, revealed c...
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