Mouse plasma glutathione peroxidase. cDNA sequence analysis and renal proximal tubular expression and secretion.
作者： R L Maser , B S Magenheimer , J P Calvet
DOI: 10.1016/S0021-9258(18)47126-8
关键词: Epididymis 、 GPX3 、 Northern blot 、 Biology 、 Fetal Kidney 、 Messenger RNA 、 Glutathione peroxidase 、 Kidney 、 In situ hybridization 、 Molecular biology
摘要: A mouse kidney cDNA isolated by differential screening was found to be highly homologous rat, human, and bovine plasma glutathione peroxidase (GPx) sequences. Analysis of the full-length coding region sequence demonstrated an in-frame selenocysteine-encoding opal codon putative signal sequence, suggesting that represents homolog GPx. The level expression GPx in various tissues during development investigated Northern blot analysis. Plasma mRNA observed very abundant compared with placenta, epididymis, intestine, lung, heart, testis, ovary, salivary gland, spleen, thymus, stomach, brain, fetal could not detected pancreas or liver except from pregnant mice. In addition, levels were shown increase postnatal kidney. situ hybridization localized proximal tubules, while primary cell culture is synthesized secreted tubular epithelial cells. relative abundance suggests tubules may source enzyme detectable further has important function protecting oxidative damage.
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