siRNA targeting VEGF inhibits hepatocellular carcinoma growth and tumor angiogenesis in vivo.

摘要: Background/Aims We have investigated whether siRNA targeted against VEGF inhibits functional properties of endothelial cells in vitro and HCC tumor growth blood vessel formation vivo . Methods The influence siRNA-VEGF on cell proliferation, apoptosis tube were analyzed Antitumoral effects examined an orthotopic model after ex transfer or intraperitoneal treatment siRNA, respectively. Intratumoral microvessel density was assessed by CD31 staining. Results expression inhibited Hepa129 70% SVEC4-10 48% within two days transfection. In , proliferation reduced 23% 38%, Interference with signaling demonstrated pAKT hepatoma cells. Tumor application 83% 63% tumors 14 days. protein both models 29% 44%. Microvessel dropped to 34% for from transfected 39% systemic treated tumors. Conclusions results show that knockdown can be associated decreased