NFκB Activation in Mouse Pituitary: Comparison of Response to Interleukin-1β and Lipopolysaccharide
作者： Patricia Parnet , Françoise Pousset , Sophie Laye
DOI: 10.1046/J.1365-2826.2003.01013.X
关键词: Internal medicine 、 NFKB1 、 Endocrinology 、 Interleukin 、 Somatotropic cell 、 Glial fibrillary acidic protein 、 Biology 、 Receptor 、 Anterior pituitary 、 Antibody 、 Knockout mouse 、 Endocrinology, Diabetes and Metabolism 、 Cellular and Molecular Neuroscience 、 Endocrine and Autonomic Systems
摘要: The mouse anterior pituitary contains both types of interleukin (IL)-1 receptors, IL-1 receptor type I (IL-1RI) and II (IL-1RII). These receptors are expressed mainly on somatotroph cells. In the present study, ability to respond in vivo or lipopolysaccharide (LPS) was demonstrated by measuring, with an electrophoretic mobility shift assay, presence active NF kappa B complex cell nuclei from pituitaries mice injected intraperitoneally recombinant rat-IL-1 beta LPS. Using immunohistochemistry antibody directed against p65 subunit, a rapid transient response LPS observed. This predominantly glial fibrillary acidic protein (GFAP)-positive cells F4/80-labelled posterior 15 min after stimulation became faint 2 h. comparison, early strong treatment localized into cells, GFAP positive pituitary. Activation no longer apparent IL-1RI knockout mice, confirming that this is essential for transduction signal pituitary, but remained treatment. addition, we investigated effect target genes measuring mRNA proteins synthesis growth hormone (GH), IL-6 IL-1ra plasma. shown induce failed regulate GH contents release. data suggest able systemic infection via cytokine-mediated responses transduced IL-1.
sci-hub.se 参考文章(58)
del Rey A, Besedovsky Ho, Immune-neuroendocrine circuits: integrative role of cytokines. Frontiers in Neuroendocrinology. ,vol. 13, pp. 61- 94 ,(1992)
Christoph J. Auernhammer, Martin Bidlingmaier, Christian J. Strasburger, Growth Hormone and the Immune System Springer US. pp. 189- 225 ,(1999) , 10.1007/978-1-4615-5163-8_13
D Giulian, J Woodward, DG Young, JF Krebs, LB Lachman, Interleukin-1 injected into mammalian brain stimulates astrogliosis and neovascularization. The Journal of Neuroscience. ,vol. 8, pp. 2485- 2490 ,(1988) , 10.1523/JNEUROSCI.08-07-02485.1988
K L Stocking, P J Morrissey, C R Willis, K Charrier, M B Glaccum, J J Peschon, D J Livingston, C Maliszewski, J L Smith, Phenotypic and functional characterization of mice that lack the type I receptor for IL-1. Journal of Immunology. ,vol. 159, pp. 3364- 3371 ,(1997)
O Marelli, A Senik, N Haeffner-Cavaillon, F Hirsch, O Déas, N Thieblemont, S D Wright, A Haeffner, B Charpentier, Inhibitory effect of growth hormone on TNF-alpha secretion and nuclear factor-kappaB translocation in lipopolysaccharide-stimulated human monocytes. Journal of Immunology. ,vol. 158, pp. 1310- 1314 ,(1997)
L K Borysiewicz, S Lefort, N Vita, S Richards, R Reeb, M O Labéta, P Ferrara, P Sozzani, Detection and biochemical characteristics of the receptor for complexes of soluble CD14 and bacterial lipopolysaccharide. Journal of Immunology. ,vol. 158, pp. 3457- 3462 ,(1997)
C K Edwards, S M Ghiasuddin, L M Yunger, R M Lorence, S Arkins, R Dantzer, K W Kelley, In vivo administration of recombinant growth hormone or gamma interferon activities macrophages: enhanced resistance to experimental Salmonella typhimurium infection is correlated with generation of reactive oxygen intermediates. Infection and Immunity. ,vol. 60, pp. 2514- 2521 ,(1992) , 10.1128/IAI.60.6.2514-2521.1992
P. N. Moynagh, L. A. J. O'neill, D. C. Williams, Activation of NF-kappa B and induction of vascular cell adhesion molecule-1 and intracellular adhesion molecule-1 expression in human glial cells by IL-1. Modulation by antioxidants. Journal of Immunology. ,vol. 153, pp. 2681- 2690 ,(1994)
Whiteside, Quan, Herkenharn, Induction of pituitary cytokine transcripts by peripheral lipopolysaccharide. Journal of Neuroendocrinology. ,vol. 11, pp. 115- 120 ,(2001) , 10.1046/J.1365-2826.1999.00297.X
H Kimata, A Yoshida, Differential effect of growth hormone and insulin-like growth factor-I, insulin-like growth factor-II, and insulin on Ig production and growth in human plasma cells Blood. ,vol. 83, pp. 1569- 1574 ,(1994) , 10.1182/BLOOD.V83.6.1569.1569