Four carcinoembryonic antigen subfamily members, CEA, NCA, BGP and CGM2, selectively expressed in the normal human colonic epithelium, are integral components of the fuzzy coat.

摘要: To elucidate which of the seven transcriptionally active genes carcinoembryonic antigen (CEA) subfamily are expressed in human colon, we first examined mRNA expression using reverse transcriptase PCR. The result showed CEA, nonspecific crossreacting 50/90 (NCA), biliary glycoprotein (BGP), and gene family member 2 (CGM2) mRNAs were colon. determine cellular sources these members within normal colonic mucosa, situ hybridization immunocytochemistry then performed. CEA NCA clearly detectable cytoplasm columnar goblet cells at free luminal surface upper crypts with low mid crypt base. In contrast, BGP CGM2 restricted only to third surface. Colon epithelium NCA, coincided that corresponding mRNAs. Ultrastructurally, localized mainly apical glycocalyx, fuzzy coat, cells. Interestingly, molecules different microdomains coat. Furthermore, was highly coat cryptal caveolated As integral components can hardly function as intercellular adhesion molecules; they possibly play an important role epithelial-microbial interactions.