Molecular cloning of nonspecific cross-reacting antigens in human granulocytes.

摘要: To clarify the molecular structures of nonspecific cross-reacting antigens (NCAs), a family glycoproteins antigenically related to carcinoembryonic antigen (CEA), in human granulocytes, we have screened cDNA library leukocytes using probe for N-terminal domain (domain-N) NCA-50, an NCA species tumor cells. In 95 positive clones randomly selected, identified six or NCA-related including biliary glycoprotein protein a, and W272 (CGM6) which previously been reported, three new clones, W236, W264, W282, encoding novel species. W236 W264 consist domain-N, putative transmembrane domain, possible cytoplasmic domain. The domain-N is 89% similar that NCA-50 at amino acid level, whereas only 49 43% those pregnancy-specific beta 1-glycoprotein-11 (PSG11), respectively, indicating belongs subfamily within CEA family. third clone W282 encodes consisting virtually identical short hydrophilic C-terminal seem be derived from single gene by alternative splicing RNA. These are particularly unique respect they lack repetitive immunoglobulin-related domains universally found members. biochemical immunochemical properties recombinant proteins these however, did not coincide with granulocytes suggesting that, there exist least 12 whose expression under complex control.