Droplet-based lipid bilayer system integrated with microfluidic channels for solution exchange

摘要: This paper proposes a solution exchange of droplet-based lipid bilayer system, in which the inner droplet is replaced for purpose efficient ion channel analyses. In our previous report, we successfully recorded conductance alpha-hemolysin membrane using contact method that can create spontaneous at interface contacting droplets; this widely used as highly preparing planar membranes. When only pipetting droplets solution, However, drawback systems their inability to within droplets. To study effect inhibitors and promoters channels drug discovery, it would be beneficial conduct introduce proteins apply or wash-out chemicals. study, propose allows via microfluidic channels. We experimentally numerically investigated stability with respect exchanging flow rates, then demonstrated binding assay an one its blockers. The system was conducted less than 20 s without rupturing membrane. believe proposed will enhance efficiency